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DNA lab: useful links

This is a list of additional resources for the DNA lab theme and information sheets.

DNA extraction

DNA extraction animation
An interactive showing the steps of DNA extraction, from Learn.Genetics – an online Genetics Science Learning Centre run by the University of Utah.
http://learn.genetics.utah.edu/content/labs/extraction/

Restriction enzymes

Examples of restriction enzymes and their recognition sites
This is an animation showing how restriction enzymes work.
www.dnalc.org/resources/animations/restriction.html

A closer look at restriction enzymes
A detailed explanation of restriction enzyme activity, including specific examples, developed by Colorado State University.
www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/index.html

DNA ligation

A closer look at ligation
A detailed explanation of ligation, including scientists proving that DNA fragments have been joined together by ligation. Developed by Colorado State University.
www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/ligation.html

Bacterial transformation

Bacterial cell structure
Get to know more about what’s inside a bacterial cell, from CELLS alive!
www.cellsalive.com/cells/bactcell.htm

DNA, plasmids and bacterial transformation
Examples of plasmids that are used in the laboratory for bacterial transformation, from Dr John Kimball at Harvard University.
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html

Bacterial transformation
A step-by-step guide to doing a bacterial transformation in the lab, from Plattsburgh State University of New York.
http://faculty.plattsburgh.edu/donald.slish/Transformation.html

Making recombinant DNA in the classroom
A cut and paste activity to demonstrate recombinant DNA in the classroom.
www.biologyjunction.com/ecoli%20insulin%20factory.pdf
A video resource demonstrating how to make recombinant DNA.
www.pbslearningmedia.org/resource/biot11.sci.life.gen.labrecomb/recombinant-dna-lab-in-the-classroom-and-in-real-world-research

Polymerase chain reaction (PCR)

Polymerase chain reaction animation
An interactive showing the steps of PCR, from Learn.Genetics – an online Genetics Science Learning Centre run by the University of Utah.
http://learn.genetics.utah.edu/content/labs/pcr

Gel electrophoresis

Gel electrophoresis (Colorado State University Biotechnology Index)
This site provides an excellent explanation of gel electrophoresis and has a link to a very good interactive activity for senior biology students.
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/agardna.html

DNA sequencing

The University of Michigan DNA Sequencing Core
An excellent explanation of the Sanger (di-deoxy) DNA sequencing method, with very clear diagrams.
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html

Microarrays/DNA chips

Anatomy of a comparative gene expression study
Detailed explanations about how microarrays are made and used, in very user-friendly language.
www.cs.wustl.edu/~jbuhler/research/array/

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